Combi Flash EX Spezifikationen PDF herunterladen (Seite 11)

High Performance Liquid Chromatography (HPLC)

Model:

Serial Number:

Before switching on the instrument

1. Sign-in with date, name and sample information on the HPLC log book

2. Check solvent levels in each container at the top of the DGU-20As degasser and make

sure that the tube filters are fully immersed in the liquids. If the solvent levels are low,

sonicate HPLC grade solvent in a separate container for about 10 min and refill the

containers with appropriate solvents

3. Check waste bottle under the table and make sure it is properly labeled and not

overflowing. If the waste bottle is close to being full, replace with an empty waste bottle

and label

Switching on the instrument

4. Instrument: Turn on the LC-20AD liquid chromatograph, SIL-20A autosampler, RID-

10A by refractive index detector, SPD-20AV UV detector and CTO-20AC column oven

by switching on the power buttons

5. Instrument: Turn on the communicator buzz module

6. Software: Click on the software “LCsolutions”>Operations>Instrument 1>Ok

7. Wait for the beep (software connecting to the instrument)

Purging and starting the pump

8. Start pump flow on the software by clicking on the table on the right and changing Pump

A. Flow to 1.00 mL/ min

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Combi Flash EX Spezifikationen Seite 11