Combi Flash EX Spezifikationen PDF herunterladen (Seite 12)

9. Instrument: On the LC-20AD liquid chromatograph, open the drain by turning the knob

anti-clockwise, and press purge. When purging is complete, close the drain by turning the

knob clockwise

10. Instrument: Press pump to turn on the pump and allow for pressure to stabilize and record

the final pressure (allow solvent flow for about ~ 10 min and monitor pressure)

Setting parameters for the analysis

11. Software: Click on Data Acquisition tab and set parameters such as LC stop time, Pump

mode, Solvent compositions, Detector wavelengths, Oven temperature etc. Save method

by clicking on File>Save method file as>

Sample preparation and using the autosampler

12. Instrument: Filter sample through a Nylon 0.45 micron membrane, place sample in blue-

capped Prominence vial (minimum sample level has to be above the middle white marker

on the vial), insert samples in the sample tray by opening the autosampler door (left

bottom) and pulling out the tray—note the vial position number of each sample on the

tray. Reposition the tray by pushing it in—make sure it clicks in, and close the

autosampler door

Starting the analysis

13. Software: Click on Batch Table tab and enter sample information—Vial number (from

the autosampler tray), Sample name, Injection volume…etc. To choose the previously

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Combi Flash EX Spezifikationen Seite 12