Combi Flash 7650 Series Bedienungsanleitung PDF herunterladen (Seite 116)

CombiFlash Torrent® User Manual

4-36

If you selected Solid (Pause), the module equilibrates

the column with the starting %B and then waits while

you prepare the solid sample load cartridge. Place the

cartridge on the module and click OK to continue.

If you selected Solid, the module equilibrates the

column and immediately starts the separation.

If you selected None (on column), the module skips the

equilibration and immediately starts the separation.

If you selected Liquid, the module equilibrates the

column and then waits. When instructed, perform either

a Syringe Injection or use the Sample Load Pump:

Syringe Injection:

a. Connect the Liquid Injection Accessory from the

accessory kit to the Sample Injection Port.

b. Place the syringe on top of the Liquid Injection

Accessory valve.

c. Open the valve by turning the handle so it is in line

with the tubing.

d. Inject the liquid sample mixture.

e. Close the valve by turning the handle so that it is

perpendicular to the tubing. Remove the syringe.

f. Inject a chase solvent by connecting a syringe loaded

with a chase solvent to the Liquid Injection

accessory’s luer fitting.

g. Open the valve by turning the handle so it is in line

with the tubing.

h. Inject at least 10 mL of the chase solvent.

i. Close the valve by turning the handle so that it is

perpendicular to the tubing. Remove the syringe.

j. Click the OK button to continue the run.

Sample Load Pump:

a. Connect the outlet tubing from the Sample Load

pump to the Torrent module’s Sample Injection Port.

b. Prepare the Sample Load pump to receive the sample:

If the sample mixture will be transferred to the pump,

insert the empty cartridge body onto the pump inlet.

Then, fill the cartridge with a measured mixture

volume.

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Combi Flash 7650 Series Bedienungsanleitung Seite 116