Combi Flash 7650 Series Bedienungsanleitung PDF herunterladen (Seite 59)

Section 2 Preparation

2-39

10.After equilibration, the Torrent will pause for sample

injection. Draw 2 mL of the dissolved Test Mix B solution

into a syringe and inject it:

a. Place the syringe on top of the Liquid Injection

Accessory valve.

b. Open the valve by turning the handle so it is in line with

the tubing.

c. Inject the liquid.

d. Close the valve by turning the handle so that it is

perpendicular to the tubing. Remove the syringe.

Note

A chase solvent forces the sample through the automated

injection valve.

11.Inject 10 mL of ethyl acetate as a chase solvent:

a. Draw 5 mL of ethyl acetate into the syringe and inject

this as described in the sub-steps of step 10 above.

b. Repeat step a for a second 5 mL injection (10 mL total).

12.Touch the OK button to continue the run.

13.While the Torrent is operating, observe the Fraction

Collector/Fractionation Valve operation. Peaks should be

cut and collected in tubes/containers. Waste fluids should

be diverted to the waste container.

14.When the Torrent finishes the run, review the resulting

chromatogram which should meet the following criteria

(refer to Figure 2-22):

• Baseline must not rise more than 0.1 AU during

separation.

• Height of Peak 2 must be higher than Peak 3.

• Retention time of Peak 2 (time that the top of the

second peak occurs) must be between 5.5 and 7.5

column volumes.

If the criteria is not met, repeat the verification steps. If the

results still do not meet the listed criteria, contact your

Teledyne Isco representative.

1 2 ... 54 55 56 57 58 59 60 61 62 63 64 ... 158 159

Keine Kommentare

Combi Flash 7650 Series Bedienungsanleitung Seite 59