Combi Flash 7650 Series Bedienungsanleitung PDF herunterladen (Seite 75)

Section 3 Operation

3-13

3. Open the valve.

4. Inject the liquid sample mixture.

5. Close the valve and remove the syringe.

Note

Chase solvent clears the Liquid Injection Adapter tubing and

forces the liquid compound mixture onto the column. Without the

chase solvent, some of the sample will remain in the tubing before

the automated injection valve and will not be separated. This small

amount of sample may also cross-contaminate the next purifica-

tion run. The chase solvent should be strong enough to prevent

the sample from precipitating (or crashing), but not so strong as to

adversely affect the separation of compounds. Often, the initial liq-

uid phase solvent is a practical chase solvent.

6. Inject a chase solvent:

a. Connect a syringe loaded with a chase solvent to the

Liquid Injection Adapter’s luer fitting.

b. Open the valve by turning the handle so it is in line with

the tubing.

c. Inject at least 10 mL of the chase solvent.

d. Close the valve by turning the handle so that it is

perpendicular to the tubing. Remove the syringe.

7. Click the OK button to continue the run.

Note

Chase solvent will remain in the line up to the automated injection

valve. Keep in mind that this solvent may affect the next injection.

3.4.2 Sample Load Pump

1. Connect the outlet of the Sample Load pump to the

Torrent’s Sample Injection Port.

Note

To bypass the injection valve, the sample may also be pumped

onto to the column at the upper column mount. If this method is

preferred, use the Direct Injection Adapter on the upper column

mount to ease tubing changes. (See section 3.1.3.)

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Combi Flash 7650 Series Bedienungsanleitung Seite 75