Combi Flash 7650 Series Bedienungsanleitung PDF herunterladen (Seite 124)

CombiFlash Torrent® User Manual

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button. The settings will revert to the original factory settings.

Click the Save button to save the settings for future use.

4.4.5 Defining a Gradient

The simplest way to change the gradient is to click and drag

the inflection points that define the shape of the gradient. Add

a point by clicking the Insert button, then click the gradient

curve to add a point. You can then drag the new point to the

desired location. Delete a point by clicking the Delete button

and then clicking the undesired inflection point.

The above methods work in the M

AIN WINDOW [4.3.1] and

ETHOD EDITOR [4.3.2] window. Alternatively, you can modify,

add, insert, and delete points using a tabular view on the

Method Editor window. Click the Edit Gradient button to open

this view, then use the controls to modify the settings. Click

the Edit Gradient button again to close the table view.

PeakTrak can also optimize a gradient from your TLC results.

To use this feature, select a single solvent system and run two

TLC plates, each with a different concentration of solvent. For

example, run one plate at 1:1 hexane:ethyl acetate, the other

at 1:3 hexane:ethyl acetate. If the resulting Rf values are

between 0.2 and 0.8, you can enter the TLC data in the

RADIENT OPTIMIZER [4.3.3] window. When you accept the data,

PeakTrak will generate a gradient curve to separate the target

compound from the nearest impurity. When using optimized

gradients, PeakTrak may automatically insert an isocratic

hold to further increase the resolution.

4.4.6 Real-time Gradient Editing

The gradient shape can be changed during the run. Any time

after the fraction collector has positioned the drop former over

the first tube, click and drag the points on the gradient plot

area of the Main window. You can also add or remove points by

clicking the Insert or Delete point buttons. The Insert button

allows you to add a single point when you click on the gradient

profile. Repeat this action to add more points. The Delete

button allows you to remove a single point when you click on

it.

Note that only the portion of the gradient that has not yet

occurred during the run can be modified.

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Combi Flash 7650 Series Bedienungsanleitung Seite 124