Combi Flash 7650 Series Bedienungsanleitung PDF herunterladen (Seite 118)

CombiFlash Torrent® User Manual

4-38

4.4.2.1 Basic method settings

The basic settings allow you to control the %B gradient mix,

flow rate, solvents, and run length. These settings are part of

the M

AIN WINDOW [4.3.1]. These settings are the most

frequently changed settings when developing or improving

purification methods.

The %B gradient mix and run length are shown on the plot

area. Refer to the D

EFINING A GRADIENT [4.4.5] topic for more

details. The other settings are:

• Flow Rate — When using the Isco default column

methods, the flow rate is automatically set to the

optimum flow rate of the column. If using a non-Isco

column, refer to the manufacturer’s column

documentation to determine the best flow rate for the

column. Note that if the column is not detected by the

module, the operating pressure is limited to 50 psi.

• Solvent A and B — Select the desired solvents from the

lists. The solvents are those defined by the

ONFIGURATION [4.3.10] window.

4.4.2.2 Advanced method settings

The METHOD EDITOR [4.3.2] window gives you access to

advanced settings such as equilibration volume, initial waste,

air purge, peak detection, and peak collection mode.

Equilibration Volume Column equilibration is optional, but

you may find that separations are more effective if the column

is equilibrated before injecting the sample. The recommended

equilibration volume is at least three column volumes of the

starting mobile phase.

RediSep Rf Silica gel column volumes are:

• RediSep Rf 80 g = 125 mL

• RediSep Rf 120 g = 192 mL

• RediSep Rf 220 g = 334 mL

• RediSep Rf 330 g = 443 mL

• RediSep Rf 750 g = 1.2 L

• RediSep Rf 1.5 kg = 2.4 L

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Combi Flash 7650 Series Bedienungsanleitung Seite 118