Combi Flash 7650 Series Bedienungsanleitung PDF herunterladen (Seite 79)

Section 3 Operation

3-17

Note

When you click the Next Tube button, the system immediately

marks the tube advance on the chromatogram. However, the

system delays the movement of the drop former over the tubes.

This delay accounts for the volume held in the tubing between the

detector and the drop former, sometimes called the “delay

volume.” It is important to remember that the chromatogram

displays activity in real time while the fraction collector activity lags

behind. The duration of this delay will vary with the flow rate.

• Rewind — This button is active when the system has

completed the run, or if the run was stopped by clicking

the Stop button. The Rewind button returns you to the

Main window with the current method.

If you have stopped the run before its programmed run

length has elapsed, clicking the Rewind button changes the

current method to the new, shorter run length. This modi-

fied method is ready for the next run, or can be saved for

future runs.

• Fast Forward — Click this button to jump to the next

step of a run.

Once you have started a run, the system performs several

steps. The first step is to deliver solvents using the pro-

grammed gradient for the entire run length. When this step

is complete, the system will purge the column with air and

clean the injection valve. Clicking the Fast Forward button

will cause the system to skip any remaining time in the

current step and advance to the beginning of the next step.

Unlike the Rewind button, clicking the Fast Forward but-

ton to complete the run will not modify the current

method’s run length setting.

Note

Solvent may be under pressure. Do not remove a column or solid

load cartridge until the pressure gauge on the touch screen to

return to 0 psi.

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Combi Flash 7650 Series Bedienungsanleitung Seite 79